Composite

Part:BBa_K1039007

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-12)

Bxb1 Integrase and Recombination Directionality Factor expression cassette

A strong promoter and strong RBS (BBa_K608002) drive expression of Bxb1 integrase and Bxb1 recombination directionality factor (RDF) (BBa_K1039003 and BBa_K1039006). The RBS (BBa_B0034) is associated with RDF.

Together, Bxb1 integrase and RDF (BBa_K1039003 and BBa_K1039006) catalyze site-specific recombination between Bxb1 attR (BBa_K1039033) and attL (BBa_K1039034) sites.

They will also allow flipping of Bxb1 invertible promoter switches BBa_K1039004 and BBa_K1039004 from RL state to PB state.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 253
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 527
    Illegal XhoI site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1166
    Illegal NgoMIV site found at 1253
    Illegal AgeI site found at 303
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1361


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